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. 2016 Apr 18;35(44):5735–5745. doi: 10.1038/onc.2016.107

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Copyright © 2016 Macmillan Publishers Limited

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Effects of monocytes/macrophages and granulocytes on S100A8 and S100A9 expression. Expression of (a) S100a8 and S100a9 mRNA in MC38 and LLC cells was assessed by quantitative PCR (qPCR) after culture in tumor-infiltrating monocyte/macrophage- or granulocyte-conditioned media. Expression of (b) S100a8 and S100a9 mRNA in MC38 and LLC cells was also assessed after culture in conditioned media derived from naive monocytes/macrophages or granulocytes. (c) TNFα expression in conditioned media derived from tumor-infiltrating (tumor monocyte CM) and naive (naive monocyte CM) monocytes/macrophages was assessed by enzyme-linked immunosorbent assay. (d) S100a8 and S100a9 mRNA in MC38 and LLC cells was assessed by qPCR untreated or after treatment with 100 ng/ml TNFα. (e) S100a8 and S100a9 mRNA in MC38 and LLC cells was assessed by qPCR after culture in control or tumor-infiltrating monocyte/macrophage-conditioned media with or without anti-TNFα (TNFi) inhibitory antibodies. For all studies, three independent experiments were performed, *P<0.05, **P<0.01 compared with controls.