Figure 3.

Histone deacetylase 6 (HDAC6) inhibition suppressed the growth factor–stimulated vascular smooth muscle cell (VSMC) proliferation and migration and blocked cell-cycle progression in vitro. A, VSMCs were grown in a 96-well plate in the absence or presence of tubastatin A (Tuba, 10 μM or 20 μM) with platelet-derived growth factor BB (PDGF-BB; 20 ng/mL) for 48 h. Data are mean±SEM (n=7). Representative images (B) and quantitative data (C) of effect of tubastatin A on the PDGF-BB–induced migration of VSMCs. Mouse VSMCs were scratched with a 1-mL pipet tip and cultured for 36 h in DMEM containing PDGF-BB (20 ng/mL) with or without tubastatin A (Tuba, 10 μM and 20 μM; n=7). The dotted lines define the areas lacking cells; ANOVA and Bonferroni post hoc tests. D-G, Representative images (D) and the quantitative data (E,F, and G) show the distribution of cells in G1 (E), S (F), and M (G) stage expressed as percentage of total cells. Data are mean±SEM (n=4), Student unpaired t test.