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Composition of the 6-plex assay cocktail.
| Component | Concentration |
|---|---|
| GBA-S | 500 μM |
| GALC-S | 850 μM |
| GAA-S | 350 μM |
| GLA-S | 1200 μM |
| IDUA-S | 250 μM |
| SMPD1-S | 750 μM |
| GBA-IS | 20 μM |
| GALC-IS | 10 μM |
| GAA-IS | 24 μM |
| GLA-IS | 24 μM |
| IDUA-IS | 15 μM |
| SMPD1-IS | 15 μM |
| sodium taurocholate | 28 mM |
| acarbose | 8 μM |
| D-saccharic acid-1,4-lactone | 40 μM |
| ZnCl2 | 0.6 mM |
| sodium oleate | 4.27 mM |
| N-acetyl-galactosamine | 50 mM |
| Succinic Acid | 85 mM |
| sodium hydroxide | sufficient to bring pH to 4.71 |
