Fig 3. TLR7/9 are critical for IFN-I production and host survival in the mouse model of Histoplasma infection.

Mice were intranasally infected with a sub-lethal dose of 3x10⁵ Histoplasma yeasts. (A) Kaplan-Meir survival curves of female wild-type (n = 10), IFNAR^-/- (n = 10), TLR7/9^-/- (n = 10) or PBS-treated (uninfected) (n = 4) mice. (B) Lungs, spleens and (C) brains of infected wild-type and TLR7/9^-/- mice were harvested, homogenized and plated for CFUs at the indicated days post-infection (dpi) (n = 5 mice/time-point). (D) IFN-I was measured in wild-type and TLR7/9^-/- lung homogenates using ISRE reporter cells. Each symbol represents a single mouse. All results are representative of at least three experiments. *p<0.05; **p<0.001; p values were determined by ANOVA.