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Lower concentration of TNF-α treatment for 2 h increases Nrf2 regulated antioxidant proteins in HL-1 cardiomyocytes. (A) Immunoblot analyses of Nrf2 regulated antioxidant proteins (GCLC, GCLM, G6PD, GPX1, CAT, NQO1, SOD1 and SOD2) in 2 h of TNFα treated HL-1 cells (n=3). (B) Relative intensity of the protein signal was calculated using Image-J software and normalized to GAPDH. Statistical significance was calculated by Mann Whitney test, where *p<0.05 vs control.
