TNF-α treatment for 24 h increases Nrf2 regulated antioxidant proteins in HL-1 cardiomyocytes. (A) Immunoblots showing protein expression of antioxidant enzymes in TNF-α treated HL-1 cells (GCLC, GCLM, G6PD, GPX1, CAT, NQO1, SOD1 and SOD2) for 24 h (n=3). (B) Densitometry quantification for appropriate immunoblots normalized with GAPDH intensity. Statistical significance among different treatments were analyzed by Mann Whitney test, where *p<0.05 vs control.