Fig 3. Endothelial and fibroblastic ECM-derived peptides enhance human dermal fibroblast proliferation in vitro.

Dermal fibroblasts isolated from healthy adult human donors grown in 48-well plates were treated with peptides at 100 nM every other day for a period of 5 days, and cell counts were performed on Day 5 as described in “Methods.” Relative proliferation compared with 1% FBS (control) is shown as fold of control, calculated by normalizing the mean cell count for each treatment to the mean cell count obtained for negative control, and error bars represent standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001 compared with negative control (unpaired Student’s T-test).