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. 2016 Jul 26;11(7):e0159653. doi: 10.1371/journal.pone.0159653

Fig 5. Antimicrobial activities of rMAP30-KDEL in samples of the hairy root clones.

Fig 5

The antimicrobial activities of the total proteins (160 μg) derived from the transgenic hairy root clones were assayed using E. coli (A), which were compared using an inhibition-halo plate assay as well (B). Dose-dependence of the antimicrobial activity of rMAP30-KDEL was observed using 40, 80, 120, and 160 μg of total hairy root soluble proteins against E. coli for 16 h (C). G indicates gentamycin (10 μg/disc) and C indicates 160 μg of nontransgenic hairy root total proteins (control). The diameter of the inhibition zones in millimeters is shown on the x axis and the six transgenic hairy root clones are shown on the y axis. In all cases, a P value of ≤ 0.01 was considered significant.