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. 2016 Jul 26;11(7):e0159789. doi: 10.1371/journal.pone.0159789

Fig 3. Expression of Na+/K+-ATPase α subunits in A549 cells and binding affinity of BF or BF211 to Na+/K+-ATPase α subunits.

Fig 3

(A) The results of the RT-PCR analysis of the expression levels of the α subunits of Na+/K+-ATPase in A549 cells. The data comprise the statistical results (n = 3, mean ± SEM) of three independent experiments. (B) The representative results of the western blot assay probing the protein levels of the α1 and α3 subunits of Na+/K+-ATPase in A549 cells. (C) The real time binding affinity measurements of BF or BF211 to the recombinant Na+/K+-ATPase α subunits. The binding ability between the compound and the recombinant protein is reflected in recorded response unit (RU) values.