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. 2016 Jul 26;11(7):e0159844. doi: 10.1371/journal.pone.0159844

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© 2016 Kaundal et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A) and (B) Both SEC and AUC data suggests dimeric state of CysK. (C) CdiA-CT^(M)/CysK complex elutes as a single peak when mixed 0.5:1 molar of CysK dimer:CdiA-CT^(M). Peak I, CdiA-CT^(M)/CysK complex; peak II, excess CdiA-CT^(M) (D) AUC analysis suggests 2:2 stoichiometric complex of CdiA-CT ^(M)/CysK. Peaks I and II corresponds to CdiA-CT^(M) and CdiA-CT^(M)/CysK complex, respectively. (E) The stoichiometric homogenous ternary complex is formed when preformed CdiA-CT^(M)/CdiI complex is mixed with dimeric CysK in 1:0.5 molar ratio. Peak I, CdiA-CT^(M)/CdiI/CysK complex; peak II, excess CdiA-CT^(M)/CdiI complex. (F) CdiA-CT^(M)/CdiI/CysK forms 2:2:2 stoichiometric complex as suggested by AUC. Peak I, CdiA-CT^(M)/CdiI complex; peak II CdiA-CT^(M)/CdiI/CysK ternary complex. In all the above experiments dimeric CysK concentration is used.