Table 1. Comparison of SEC and AUC data of proteins and protein-protein complexes indicating the oligomeric states and the binding stoichiometry.
MWobs and MWcal are the observed and calculated molecular weights of the sample in kDa, respectively. The percentage population of the complexes mentioned in the parentheses corresponds to the samples where complexes were prepared by mixing equimolar ratio of the protein components.
| Sample | MWcal (kDa) | SEC | AUC | ||||
|---|---|---|---|---|---|---|---|
| MWobs (kDa) | Oligomeric State/ Stoichiometry | MWobs (kDa) (% age) | Oligomeric State/ Stoichiometry | Sw (S) | Frictional ratio | ||
| CdiA-CT(M) | 26.9 | ~51 | Apparent Dimer | 26.6 ± 0.07 (84%) | Monomer | 1.7 ± 0.01 | 1.8 ± 0.01 |
| CdiI | 16.8 | ~20 | Monomer | 16.6 ± 0.02 (90%) | Monomer | 1.5 ± 0.01 | 1.5 ± 0.02 |
| CdiA-CT(M)/CdiI | 43.7 | ~67 | Heterodimer | 41.2 ± 0.12 (70%) | Heterodimer | 2.2 ± 0.13 | 1.89 ± 0.04 |
| CysK | 36.7 | ~70 | Homodimer | 73.7 ± 0.19 (84%) | Homodimer | 4.3 ± 0.06 | 1.46 ± 0.01 |
| CysK/CdiA-CT(M) | 63.6 | ~230 | Heterotetramer | 126 ± 0.92 (80%) | Heterotetramer | 4.1 ± 0.10 | 2.0 ± 0.02 |
| CysK/CdiA-CT(M)/CdiI | 80.4 | ~239 | Heterohexamer | 157.6 ± 0.52 (77%) | Heterohexamer | 5.1 ± 0.14 | 1.90 ± 0.01 |