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. Author manuscript; available in PMC: 2016 Jul 26.
Published in final edited form as: Curr Opin Chem Biol. 2015 Jun 5;28:20–28. doi: 10.1016/j.cbpa.2015.05.012

Figure 2.

Figure 2

Using sensors to screen for high yields of a desired compound. The bacterium on the left has been engineered to produce lysine (red triangle) which is sensed by a transcription factor (blue polygon) that activates the expression of GFP. After mutagenizing this strain, lysine production by different mutants can be detected via their level of GFP expression. Mutants that produce high amounts of lysine also produce high amounts of GFP and can be picked for further mutagenesis. Directly measuring lysine quantities produced by different stains using a technique like liquid chromatography mass spectroscopy (LC-MS) confirms the correlation between GFP expression and lysine production.