Table 2.

Effects of dermal exposure to DDAC on DLN cell number and lymphocyte sub-population in female Balb/c mice.

	DDAC
Parameter	0%	0.25%	0.05%	1%
DLN number (×106)	3.65 ± 0.47	15.2 ± 0.83**	15.5 ± 1.08**	17.0 ± 1.42**
B-cell number (× 106)	0.51 ± 0.09	2.8 ± 0.13**	2.8 ± 0.21**	4.0 ± 0.32**
% of DLN	14.12 ± 1.5	18.52 ± 0.27*	18.02 ± 1.45	23.80 ± 0.46**
CD4 + T-cell number (× 106)	2.2 ± 0.29	8.4 ± 0.47**	8.6 ± 0.68**	8.7 ± 0.76**
% of DLN	60.04 ± 0.96	55.28 ± 0.25**	54.98 ± 0.25**	51.02 ± 0.50**
CD8+-cells (× 106)	0.92 ± 0.11	3.7 ± 0.21**	3.5 ± 0.32**	3.9 ± 0.29**
% of DLX	25.26 ± 0.66	24.46 ± 0.22	2490 ± 0.46	22.98 ± 0.51*
Dendritic cells (× 105)	0.4 ± 0.06	1.5 ± 0.08**	2.0 ± 0.11**	1.9 ± 0.19**
% of DLN	1.18 ± 0.14	0.96 ± 0.05	1.33 ± 0.08	1.15 ± 0.09

Mice were dermally exposed to vechicle (acetone) or different concentrations of DDAC for 4 consecutive days. The mice were euthanized 6 days after the final exposure, DLN were removed, and total cells counted. Numbers and frequency of B- and T-cells, and subsets of T-cells (CD4⁺ and CD8⁺), and dendritic cells were enumerated. Values represent the means (± SE) for each group. Significantly different from acetone controls at

^*p<0.05 and

^**p<0.01