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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Stroke. 2016 Jul 7;47(8):2103–2111. doi: 10.1161/STROKEAHA.116.012763

Figure 2.

Figure 2

A, Dynamin-1 is increased more markedly in Hsp70Ko mice 3 d post insult, compared to Wt mice, whereas expression is reduced in Hsp70Tg brains (n=3/group, scale bar=50 μm). B, Immunoblots show dynamin-1 from Wt, Hsp70Ko and Hsp70Tg mouse brains 1d after dMCAO. Relative intensities of protein normalized to β-actin show that dynamin-1 was increased in Hsp70Ko compared to Wt and Hsp70Tg (n=3/group, *P<0.01, **P<0.05). C, 1 d post ischemia, brain lysates were immunoprecipitated using anti-Hsp70. Immunoblots (IB) of the immunoprecipitate (IP) showed significant levels of dynamin-1, indicating that Hsp70 associates with dynamin-1 (n=3/group, *P<0.01). Relative intensities of the immunoblots showed that Hsp70 highly associated with dynamin-1 in Hsp70Tg. D, 3 days post dMCAO, dynamin-1 (red) and Fas (green) colocalized (Merge, yellow). Fas and dynamin-1 were reduced in Hsp70Tg brains and increased in Hsp70Ko brains compared to Wt (n=3/group, scale bar=10 μm). In ischemic brain, dynamin-1 also colcalized with TUNEL-positive cells in Wt mice.

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