Figure 3.

A, 3 d post dMCAO, Fas staining was more intense after dMCAO in Hsp70Ko, but decreased in Hsp70Tg (n=3/group, scale bar=50 μm). 1 d post dMCAO, ischemic brain tissues were fractionated, and subjected to immunoblotting. Fas from membrane and cytosolic fractions increased in both fractions following dMCAO, but Fas was most increased amongst Hsp70Ko, and the least in Hsp70Tg. Fas was also higher in the membrane fraction amongst Wt and Hsp70Ko, compared to the cytosolic fraction, but decreased in Hsp70Tg (n=3/group, *P<0.01). B, Hsp70 induced in N2a cells with 17-AAG and subjected to OGD show fewer Fas positive cells by quantitative FACS compared to controls. (n=3/group, *P<0.01). C, 3d post dMCAO, caspase-8 staining was more intense after dMCAO in Hsp70Ko, but decreased in Hsp70Tg (n=3/group, scale bar=50 μm). Immunoblots show caspase-8 expression 1day after dMCAO. Caspase-8 was decreased in Hsp70Tg compared to Wt and Hsp70Ko (n=3/group, *P<0.01). D, 3 d post dMCAO, Hsp70Ko showed increased numbers of TUNEL-positive cells in the ischemic cortex when compared to Hsp70Tg. The percentage of TUNEL-positive cells normalized to total cell numbers acquired from sham show fewer TUNEL-positive cells in samples from Hsp70Tg compared to Wt or Hsp70Ko (n=5/group, *P<0.01, **P<0.05, scale bar=50μm)