Model of hypothalamic phenotype in Rax conditional knockout embryos. Schematics of gene expression territories at E12.5 in sagital cuts of a wild-type embryo (left) and a RaxKO@E8.0 embryo (right). In the embryo lacking Rax, the expression of Shh (yellow) in the ABasM is abolished, and the formation of mature neurons expressing dorsomedial hypothalamic markers (Pomc, Otp, TH, Sst; blue dots) is also abolished or greatly reduced. At the same time, the expression of ventromedial hypothalamic markers (Fgf10, Otx2, Tbx3, red) extends into dorsomedial territory (arrow).