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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: J Neurochem. 2016 Jun 18;138(3):397–406. doi: 10.1111/jnc.13676

Figure 5. Lysosomal-dependent degradation of TrkB by BDNF or NT4.

Figure 5

A surface biotinylation degradation assay was performed with cortical neurons treated for 3–4 hours with 50 ng/ml BDNF, NT4 or untreated in the presence or absence of 100 μg/ml leupeptin. a) Representative western blot with TrkB antibody. b) Quantification of an average of three independent experiments. 50 ng/ml with BDNF alone led to a degradation index of 27.0 ± 3.2%M; BDNF and leupeptin 68.5 ± 6.4 % (****p < 0.0001, Student's t test). 50 ng/ml of NT4 led to a degradation index of 68.9 ± 5.8 %, NT4 and leupeptin 90.3 ± 1.7% (*p < 0.05, Student's t test).