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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: J Neurochem. 2016 Jun 18;138(3):397–406. doi: 10.1111/jnc.13676

Figure 6. Sustained signaling mediated by BDNF or NT4.

Figure 6

a) Cortical neurons were treated for indicated times with 50 ng/ml of BDNF, NT4 or left untreated. Lysates were immunobloted for phoshpo-Akt (pAkt) and phospho-Erk (pErk). b) Cortical neurons were pre-treated with 100 μg/ml leupeptin for 1 hr. Then neurons were stimulated with 50 ng/ml of BDNF, NT4 or left untreated in the presence or absence of leupeptin for indicated times. Lysates were immunobloted for phoshpo-Akt (pAkt) and phospho-Erk (pErk). c) Quantification represents phosphorylation of Akt and Erk normalized to tubulin. Average of three independent experiments (*p<0.05, **p<0.01, Student's t test).