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HPLC chromatogram of dpCoA stock (Sigma-Aldrich, lot SLBJ2886V; 50 nmol). Green: HPLC chromatogram of AMP (20 nmol). Comparison of chromatograms indicates that the dpCoA stock contains ~2% AMP. The observation that the dpCoA stock contains ~2% AMP in the dpCoA stock accounts for the formation of pApC in reactions performed with dpCoA (Figure 1b).
