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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Br J Haematol. 2016 Apr 20;174(3):397–409. doi: 10.1111/bjh.14065

Figure 3. Melflufen triggers robust γ-H2AX foci formation in melphalan-resistant LR-5 cells.

Figure 3

RPMI-8226 and LR-5 cells were treated with 3 µM of melflufen or melphalan for 4h, and then subjected to immunofluorescence (IF) staining (anti-γ-H2AX as primary and Alexa Fluor- 488 conjugated secondary antibodies; DAPI for nuclear staining). (A) Quantitative analysis of γ-H2AX foci formation in melflufen- or melphalan-treated (3 µM) RPMI-8226 and LR-5 cells.(mean±SD; P<0.05, n=3 fields per sample) (B) Representative IF showing γ-H2AX foci formation in melflufen- and melphalan-treated LR-5 cells.