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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Br J Haematol. 2016 Apr 20;174(3):397–409. doi: 10.1111/bjh.14065

Figure 6. Analysis of melflufen or melphalan-induced DNA damage with Acridine Orange (AO).

Figure 6

(A–C) MM.1S (melflufen: 1 µM; melphalan: 3 µM), RPMI-8226 and LR-5 (melflufen:3 µM; melphalan :10 µM) cells were treated with drugs for 2h; cells were washed to remove drugs and then cultured in fresh complete medium for 48h, followed by AO staining of cells using FACS. In addition, cells were treated with drugs (as above) continuously for 48h, and then subjected to analysis with AO staining. Quantitative analysis of AO-Red Fluorescence and AO-Green Fluorescence is presented.