Figure 6.
FABP1 gene ablation alters protein levels of brain cytosolic ‘chaperone’ fatty acid and endocannabinoid binding proteins. All conditions were as in Figure 5 except that Western blot analysis was performed as described (Martin et al. 2015;Kaczocha et al. 2009) to determine protein levels of (A) FABP3, (B) FABP5, (C) FABP7, (D) SCP-2, and (E) HSP70. Insets show representative Western blot images of the respective protein (upper blot) and the gel-loading control protein (GAPDH or β-Actin, lower blot). Relative protein levels were normalized to the gel-loading control protein; values were compared to WT set to 1. Results are presented as mean ± SEM (n = 7); *, p < 0.05 for LKO vs WT.