Table 1.
Gene names, cellular locations, and oligonucleotides used as primers in amplification reactions.
| Gene | mRNA | Comments*cellular location | Amplification primers | Amplicon size (bp) | |||
|---|---|---|---|---|---|---|---|
| Product | Symbol | Reference sequence | Forward/Reverse | Targeted exons | cDNA | gDNA** | |
| Hypoxanthine guanine phosphoribosyl transferase | Hprt | NM_013556 | IS | TCAGACTGAAGAGCTACTGTAATGA/AAGTTTGCATTGTTTTACCAGTG | 3rd/4th 6th | 136 | N/A |
| TATA binding protein | Tbp | NM_013684 | IS RIA (3′') | GACCCACCAGCAGTTCAGTAG/CTCTGCTCTAACTTTAGCACCTGT | 6th 7th/8th | 136 | N/A |
| TATA binding protein | Tbp | NM_013684 | RIA (5′') | CGCAGTGCCCAGCATCA/GCATAAGGTGGAAGGCTGTTG | 1st/2nd 2nd | 154 | N/A |
| α-Tubulin | Tuba1 | NM_011653 | gDNA contamination control | GGATTCGCAAGCTGGCTG/GGGCTGGGTAAATGGAGAAC | 3rd/4th 4 h | 162 | 162 |
| RNA Polimerase II (subunit f) | Polr2f | NM_027231 | IS | GTCAGACAACGAGGACAATTTC/ATACTTGGTCATGTAAGGAGTGGT | 1st/2nd 3rd | 178 | N/A |
| Superoxide dismutase 1 | Sod1 | NM_011434 | Cytosolic | CGATGAAAGCGGTGTGCG/GCACTGGTACAGCCTTGTGTATTG | 1st2nd/3rd | 178 | N/A |
| Superoxide dismutase 2 | Sod2 | NM_013671 | Mitochondrial | GGTGGAGAACCCAAAGGAGA/TAAGCGACCTTGCTCCTTATT | 3rd/4th 4 h | 150 | N/A |
| Catalase | Cat | NM_009804 | Peroxisomal | AGAGGAAACGCCTGTGTGAG/GTAGGTGTGAATTGCGTTCTTAG | 11th12th/13th | 173 | N/A |
| Thioredoxin 1 | Txn1 | NM_011660 | Cytosolic | AGCCCTTCTTCCATTCCCT/GAACTCCCCCACCTTTTGAC | 2nd73rd 4th/5th | 152 | 152 |
| Thioredoxin 2 | Txn2 | NM_019913 | Mitochondrial | GACACCAGTTGTTGTGGACTTTC/TAGGCACAGCTGACACCTCATA | 1st 2nd/3rd | 170 | N/A |
| Thioredoxin interacting protein | Txnip | NM_023719 | Cytosolic | GTCGAATACTCCTTGCTGATCTA/TCTGGGGTATCTGGGATGTT | 5th/6th 6th | 173 | N/A |
| Thioredoxin reductase 1 | Txnrd1 | NM_001042523 | Cytosolic | GGGGAAGAAAATATTGAAGTTTACC/TGGAAGCCCACGACACGTT | 13th/14th 15th | 134 | N/A |
| Thioredoxin reductase 2 | Txnrd2 | NM_013711 | Mitochondrial | TCACAGTGCTACATAAAGATGGTA/CAGCTTGACCACCTCCTCAG | 15th/16th 17th | 192 | N/A |
| Thioredoxin reductase 3 | Txnrd3 | NM_153162 | Endoplasmic reticulum | GACTCTTTGGGGTCTCTTTAGAA/CAAAACAAGGTGTGATACACTTCC | 12th 13th/14th | 154 | N/A |
| Peroxiredoxin 2 | Prdx2 | NM_011563 | Cytosolic | GGCATTGCTTACAGGGGTC/CCACATTGGGCTTGATGGT | 4th/5th 6th | 196 | 196 |
| Peroxiredoxin 3 | Prdx3 | NM_007452 | Mitochondrial | GGATCAACACACCAAGAAAGAAT/CGGAAGGTCGTTGACACTCAG | 4th/5th 6th | 185 | N/A |
| Peroxiredoxin 4 | Prdx4 | NM_016764 | Cytosolic/Nuclear Secreted | GTATACCTTGAAGACTCAGGACATAC/CCAGCAGGGCAGACTTCTC | 4th5th/6th | 176 | N/A |
| Peroxiredoxin 5 | Prdx5 | NM_012021 | Cytosolic/Mitochondrial/peroxisomal | CACCTGGCTGTTCTAAGACCC/AGACACCAAAGAATCATCCAATAA | 2nd/3rd 5th | 224 | N/A |
| Glutamate-cysteine ligase, catalytic subunit | Gclc | NM_010295 | Cytosolic | GCCTCCTCCTCCAAACTCAGA/ATCCCCTGCAAGACAGCATC | 11th 13th | 213 | N/A |
| Glutathione reductase | Gsr | NM_010344 | Cytosolic/Mitochondrial | CCAATGTCAAAGGCGTCTATG/AGACCACAGTAGGGATGTTGTCA | 10th 11th | 153 | N/A |
| Glutaredoxin 1 | Glrx1 | NM_053108 | Cytosolic | AGCTCACCGGAGCGAGAAC/ATCTGCTTCAGCCGAGTCATC | 1st 2nd | 118 | N/A |
| Glutaredoxin 2 | Glrx2 | NM_001038592 | Cytosolic/Mitochondrial | GTCGTTTTGGGGGAAGTCTA/GGTTGCCATATTCCAGCATAT | 2nd 3rd | 182 | N/A |
| Glutathione peroxidase 1 | Gpx1 | NM_008160 | Cytosolic | CGGCACAGTCCACCGTGTAT/ATTCTTGCCATTCTCCTGGTGT | 1st 1st/2nd | 236 | 236 |
| Glutathione peroxidase 4 | Gpx4 | NM_008162 | Cytosolic/Mitochondrial | TGGTCTGGCAGGCACCAT/TGCACACGAAACCCCTGTACT | 1st 3rd/4th | 128 | 128 |
| Sulfiredoxin 1 | Srxn1 | NM_029688 | Cytosolic | AGAGCCTGGTGGACACGAT/AGCTTGGCAGGAATGGTCT | 1st 2nd | 163 | N/A |
*
IS, internal standard for normalization; RIA, RNA integrity assessment by 3 ′:5 ′ assay. Subcellular localization of protein encoded by the detected mRNA variant.
**
N/A: the primer pair does not amplify with gDNA as template, at least with a short extension time amplification reactions.