Table 2.

Gene expression of antioxidant systems in HT22 cells exposed to ethanol (0.1%) or PBS.

Ethanol vs. PBS
	Ethanol 6 h		Ethanol 24 h		Ethanol 30 h		Ethanol 48 h
Gene	Expression	p	Expression	p	Expression	p	Expression	p
CLASSICAL SYSTEM
Sod1	1.23*	0.028	1.09	0.300	1.26*	0.009	1.19	0.054
Sod2	1.15	0.275	1.18	0.070	1.41*	0.004	1.46*	0.026
Cat	0.98	0.722	0.74*	0.014	0.94	0.255	0.88	0.134
THIOREDOXIN/PEROXIREDOXIN SYSTEM
Txn1	1.09	0.486	1.01	0.990	1.26	0.119	1.08	0.469
Txn2	0.80	0.180	1.08	0.404	1.02	0.935	0.96	0.560
Txnip	1.02	0.892	0.47*	0.008	0.86	0.137	0.30*	0.017
Txnrd1	1.16*	0.050	1.27*	0.010	1.53*	0.005	1.32*	0.018
Txnrd2	0.74*	0.032	0.85	0.250	0.84	0.161	0.76	0.094
Txnrd3	0.71*	0.027	0.70*	0.044	0.68*	0.021	0.65*	0.004
Prdx2	0.75*	0.046	0.71*	0.015	0.82*	0.015	0.73*	0.015
Prdx3	0.76	0.078	0.68*	0.036	0.84	0.310	0.73*	0.029
Prdx4	0.75*	0.012	0.67*	0.039	0.78*	0.008	0.67*	0.013
Prdx5	0.73*	0.046	0.69*	0.007	0.72*	0.011	0.61*	0.019
Srxn1	1.00	0.949	1.26	0.163	1.15	0.439	1.13	0.052
GLUTATHIONE/GLUTAREDOXIN SYSTEM
Gclc	1.13	0.135	1.07	0.700	1.26*	0.013	1.25*	0.008
Gsr	1.00	0.971	1.02	0.694	1.14*	0.007	1.09	0.136
Glrx1	1.25*	0.017	1.01	0.795	1.21	0.208	0.93	0.619
Glrx2	0.94	0.475	0.87	0.408	1.03	0.687	0.87	0.069
Gpx1	1.70*	0.011	1.84*	0.015	2.04*	0.018	2.20*	0.025
Gpx4	0.96	0.799	0.97	0.699	0.94	0.864	0.85	0.394

Results correspond to the mean of four different experiments. PBS, phosphate buffer saline; p, probability value;

^*Significant differences.

Bold values indicate those genes whose expression was altered at some stage in the experiments.