Figure 2.

HMGB1 may be released from CD11b+ cells in the aged hippocampus. A–G, Nuclear colocalization of HMGB1 was quantified in the hippocampus of young and aged rats by triple-labeling DAPI and HMGB1 with either CD11b (microglia; A, B, E), GFAP (astrocytes; C, F), or NeuN (neurons; D, G; n = 8/group). Images were captured using a Nikon A1R Laser Scanning Confocal and TIRF Inverted Microscope and analyzed using Fiji (ImageJ) by a condition-blind observer. Arrows are highlighting triple-labeled cells and arrowheads represent DAPI+ cells lacking nuclear HMGB1. Data are presented as mean ± SEM. *p < 0.05.