Figure 1.

Recombinant OPN variants. (a) The figure depicts the recombinant OPN variants: OPN-FL (aa 17–314 human and aa 17–294 mouse), OPN-N including aa 17–168 (human) or 17–153 (mouse) of OPN; OPN-C including aa 169–314 (human) or 154–294 of OPN; mouse OPN-FL^mut carrying a mutated thrombin cleavage site (from R₁₅₃-S₁₅₄ to S₁₅₃-F₁₅₄). (b) Western blotting showing the recombinant proteins after purification probed with the anti-His-tag (left panel) or antibodies specific for the N (middle panel) or C-terminal portion (right panel). (c) OPN-FL but not OPN-FL^mut is cleaved by thrombin.