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. 2016 Jul 22;7:12202. doi: 10.1038/ncomms12202

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(a,b) Modulation of enzyme activity and protein degradation in S. cerevisiae. Yeast cells were transformed with F-DHFR^wt:URA3, K1:URA3, K2:URA3 under control of the copper-inducible P_CUP1 promoter. (a) Spot tests of WT, ubr1-Δ or ump1-Δ cells in serial (1:5) dilutions grown for 3 days at the indicated temperatures. Plates were grown at 17.5 °C for 5 days. ump1-Δ shows a general growth defect at elevated temperature. (b) Cycloheximide (CHX) chase of K2:URA3. Equal loading was confirmed by simultaneously probing against Cdc11 (red signal).