Table 2.
Frequencies of micronucleated erythrocytes (% MNEs) and mitotic activity (% PCE) in bone marrow of mice exposed to hydro-meth-anol extracts of Spondias mombin (MSM), Nymphaea lotus (MNL) and Lufffa cylindrica (MLC) alone and with methyl methane sulfonate MMS (Mean±SE).
| Conc. (mg/kg) | MNPCE | MSM % Amelioration | % PCE | MNPCE | MNL % Amelioration | % PCE | MNPCE | MLC % Amelioration | % PCE |
|---|---|---|---|---|---|---|---|---|---|
| DDW | 4.8±0.8 | 61.4±1.8 | 4.8±0.8 | 61.4±1.8 | 4.8±0.8 | 61.4±1.8 | |||
| DMSO | 8.8±3.4†† | 55.3±1.5†† | 8.75±1.0†† | 60.2±4.8†† | 8.75±1.0†† | 60.2±4.8 | |||
| 50 | 7.0±1.8†† | 57.1±1.6††‡ | 9.5±1.2†† | 57.1±0.8†† | 13.67±3.0††‡‡ | 64.5±3.3 | |||
| 100 | 13.7±3.8††‡‡ | 54.9±0.6†† | 13.8±0.9††‡ | 58.0±1.6†† | 17.25±2.4††‡‡ | 55.7±0.2 | |||
| 200 | 19.0±1.0††‡‡ | 54.8±0.1†† | 7.5±1.8†† | 66.0±0.7†† | 7.25±1.4†† | 55.1±0.8 | |||
| 400 | 16.0±3.5††‡‡ | 53.0±0.9†† | 11.5±2.5†† | 66.7±0.8†† | 8.0±1.0†† | 61.1±0.9 | |||
| MMS | 28.0±3.1††‡‡ | 46.3±0.6††‡‡ | 28.0±3.1†††‡ | 46.3±0.6†† | 28.0±3.1††‡‡ | 46.3±0.6 | |||
| 50+MMS | 9.0±0.7††‡** | 57.1 | 49.4±0.5†† | 12.8±1.1††** | 39.3 | 55.2±0.7†† | 6±0.0††** | 78.8 | 55.7±0.2 |
| 100+MMS | 9.0±0.7††‡** | 57.1 | 51.1±3.5††** | 8.5±1.2††** | 59.5 | 55.5±1.9† | 14.25±.1.5†‡‡** | 44.1 | 56.2±0.0 |
| 200+MMS | 11.3±0.6††‡‡** | 46.4 | 51.6±0.9††* | 6.0±1.2††** | 71.4 | 69.5±1.2†† | 6.5±1.5††** | 75.0 | 52.8±3.5 |
| 400+MMS | 9.0±3.5††‡** | 56.0 | 59.1±1.1†‡** | 6.5±1.5††** | 69.1 | 54.1±1.6 | 5.5±1.5** | 76.2 | 52.1±6.1 |
All the groups were compared with the negative and vehicle control. The antigenotoxic groups were also compared with MMS. Significant difference from distilled water group
†
-(p<0.05)
††
- (p<0.01); Significant difference from DMSO group
‡
- (p<0.05);
‡‡
- (p<0.01); Significant difference from MMS
*
-(p<0.05)
**
-(p<0.05); DDW-distilled water; MMS-methyl methane sulfonate (60 mg/kg); MNPCE-micronucleated polychromatic erythrocyte; PCE-polychromatic erythrocyte.