Table 1.
Probit transformations of percent lethality values during PbCl2 toxicity to human lymphocytes growing in DME medium, assessed by four methods, TB, AO/EB staining, MTT assay, NRU assay and comet assay.
| PbCl2(ppm) | Log10 concen-tration of PbCl2 | PL of cells by TB staining | Probits of TB staining | PL of cells by AO/EB staining | Probits of AO/EB staining | PL of cells by MTT assay | Probits of MTT assay | PL of cells by NRU assay | Probits of NRU assay | DFI (%) | Probits of DFI |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 300 | 2.47 | 8.3 | 3.6 | 12 | 3.8 | 8.6 | 3.6 | 4.5 | 3.3 | 9 | 3.6 |
| 600 | 2.77 | 19.8 | 4.2 | 25 | 4.3 | 15.8 | 3.9 | 16.9 | 4.0 | 21 | 4.2 |
| 900 | 2.95 | 24.0 | 4.3 | 36 | 4.6 | 23.2 | 4.2 | 28.1 | 4.4 | 35 | 4.6 |
| 1200 | 3.07 | 36.6 | 4.6 | 42 | 4.7 | 41.3 | 4.7 | 40.5 | 4.7 | 41 | 4.7 |
| 1500 | 3.17 | 48.4 | 5.9 | 54 | 5.1 | 49.4 | 4.9 | 51.7 | 5.0 | 55 | 5.1 |
| 1800 | 3.25 | 60.3 | 5.2 | 70 | 5.5 | 59.5 | 5.2 | 64.1 | 5.3 | 69 | 5.4 |
| 2100 | 3.32 | 73.5 | 5.6 | 82 | 5.9 | 68.1 | 5.4 | 67.5 | 5.4 | 83 | 5.9 |
| 2400 | 3.38 | 85.0 | 6.0 | 89 | 6.2 | 86.3 | 6.1 | 79.8 | 5.8 | 91 | 6.3 |
| 2700 | 3.43 | 92.2 | 6.4 | 94 | 6.5 | 96.1 | 6.7 | 91.1 | 6.3 | 96 | 6.7 |
| 3000 | 3.47 | 100 | - | 100 | - | 98.6 | 7.1 | 93.3 | 6.4 | 100 | - |
| 3300 | 3.51 | - | - | - | - | 100 | - | 98.9 | 72 | - | - |
Note: TB, Trypan blue; AO/EB, Acridine orange/ethidium bromide; DMEM, Dulbecco's modified Eagle's medium; MTT, 3-[4, 5- dimethylthiazol-2-yl] 2,5-diphenyl tetrazolium bromide; NRU, Neutral red uptake assay; PL, Percent lethality; DFI, DNA fragmentation index; -, Not applicable. Experiments were repeated thrice and the last data set was presented.