Table 2. SAXS data-collection and scattering-derived parameters.
| SAXS analyses | |
|---|---|
| Data-collection parameters | |
| Instrument | P12 beam line (PETRA-III, DESY) |
| Wavelength (nm) | 0.124 |
| s range (nm−1) | 0.02–4.8 |
| Exposure time (s) | 0.045 |
| Concentration range (mg/mL) | 0.5–2.0 |
| Temperature (°C) | 20 |
| Structural parameters | |
| I(0) (relative) [from p(r)]* | 63,360.0 |
| Rg (nm) [from p(r)] | 4.93 |
| I(0) (relative) [from Guinier]* | 62,494.4 ± 124.65 |
| Rg (nm) [from Guinier] | 4.74 ± 0.33 |
| Dmax (nm) | 20.0 |
| Porod volume estimate (nm3) | 519.79 |
| Dry volume of a monomer calculated from sequence (nm3) | 67.56 |
| Molecular mass determination | |
| Molecular mass Mr (kDa) [from I(0) using p(r)] | 287.8 |
| Molecular mass Mr (kDa) [from I(0) using Guinier] | 283.8 |
| Calculated monomeric Mr from sequence | 55.8 |
| Software employed | |
| Primary data reduction | PRIMUS |
| Data processing | PRIMUS |
| Ab initio analysis | GASBOR |
| Validation and averaging | PRIMUS |
| Rigid-body modelling | BUNCH |
| Computation of model intensities | CRYSOL |
| Three-dimensional graphics representation | PyMOL |
*After normalization to a concentration of 1 mg/mL.