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. 2016 Jul 27;6:30377. doi: 10.1038/srep30377

Figure 3. Effect of RNaseH1 knockdown on Acsl1 gapmer-derived hepatotoxicity.

Figure 3

Acsl1 gapmer (10 mg/kg) was subcutaneously administrated 2 days after injection of siRNA-invivofectamine complex, and the liver and plasma were isolated on day 10 after ASO administration. Plasma ALT level (a); plasma AST level (b); expression level of RnaseH1 mRNA (c); Western blot analysis of RnaseH1 and Acsl1 protein (d). Two homogenates of liver in each group were analysed using β-actin as internal standard. Expression level of RnaseH1 mRNA (e) and RnaseH2a mRNA (f). In situ detection of apoptosis (g). Liver sections of mice that received both siRNA and Acsl1 gapmer was subjected to TUNEL assay and observed under confocal laser microscopy (TM; transmission, Scale bar: 5 μm). (n = 4, mean + S.D., *p < 0.05, Mann–Whitney U test, vs si-N.C. + gapmer).