Figure 1. Demonstration of the contrast improvement in X-ray micro-radiography between a native sample and ethanol preserved samples.

X-ray radiography of a native mouse heart (A) and samples preserved in 50% ethanol solution (B), 97% ethanol solution (C) and the ethanol series of increasing concentrations (D). Acquisition parameters: Tube voltage 60 kV, current 120 μA, acquisition time 40 s.