Figure 4. Allele typing of transplant-associated loci using sequence specific typing (genomic DNA) compared to single chromosome amplification and sequencing of the isolated parental chromosome 6 samples.

Alleles were confirmed by sequencing. The loci used are within exon 2 and 3 of HLA Class 1 genes (HLA-A, HLA-B and HLA-C) and HLA Class II genes (HLA-DRB1, HLA-DQA1, HLA-DQB1, HLA-DPA1 and HLA-DPB1). The percentage values are the percentage of the gene/feature covered by the aligned sequence data. Therefore, it is possible for exon 2 and 3 to be 100% covered, but the gene to only be 89.7% covered, due to poor coverage in another part of the gene.