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. 2016 Jul 27;6:30374. doi: 10.1038/srep30374

Figure 3. Impact of exchanging 5′ CP sequence between genotype B2 and C2 clones on ENII+CP activities.

Figure 3

The ENII+CP reporter constructs of three B2 clones (A,C) and three C2 clones (B,D) were transfected to Huh7 cells (A,B) or HepG2 cells (C,D), and luciferase activities were measured 2 days later. W: original (WT) clone. P1, P2, and P3: converting B2 sequences at 1633/1635/1636/1652, 1673, and 1730 into C2 consensus sequences; P1′, P2′, and P3′: converting C2 sequences at these positions into B2 consensus sequences (see Table 1). The results were based on 3 repeat experiments. *P < 0.05.