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. 2016 Jul 27;6:30374. doi: 10.1038/srep30374

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The name above the line indicates the original clone and their genotype. The name below indicates the source of core promoter. P1: G1633A/A1635G/A1636T/G1652A. P1′: A1633G/G1635A/T1636A/A1652G. CP: core promoter. Huh7 cells transiently transfected with dimeric HBV DNA constructs were harvested 3 days later. (A) Northern blot of HBV RNAs using GAPDH as a loading control. (B) Southern blot of replicative DNA; (C) Western blot of core protein with GAPDH serving as a loading control.