Table 3. Amounts of calculation for aTL .
Oligomer | Molecular weight (MW) | Weight of telomere standard and 36B4 (g) | Number copies of 36B4 | Number molecules of oligomer in TEL STD A | Amount of telomere sequence in TEL STD A (kbp) | SCG STD A |
(TTAGGG)14 | 26667.2. | 2.6667 × 104/6.02 × 1023= 0.44 × 10-19 | - | 60 × 10-12/0.44 × 1019=1.36×109 | 1.36 × 109× 84= 1.18 × 108 |
- |
synthesized 36B4 oligomer standard | 23268.1 | 2.32681 × 104/6.02 × 1023= 0.38 × 10-19 | 200 × 10-12/0.44 × 10-19= 5.26×109 | - | - | 2.63 × 109 |