Table 2.
Factor | N | Content of Isoflavones mg·g−1 | ||||||
---|---|---|---|---|---|---|---|---|
Biochanin A | Formononetin | Genistein | Ononin | Sissotrin | Daidzein | Daidzin | ||
Species | ||||||||
Trifolium pratense L. | 12 | 3.697 ± 1.40 a | 4.315 ± 1.55 a | 0.177 ± 0.06 a | 0.943 ± 0.35 a | 0.462 ± 0.20 a | 0.232 ± 0.11 a | 0.185 ± 0.07 a |
Medicago sativa L. | 12 | 0.105 ± 0.11 b | 0.141 ± 0.12 b | 0.014 ± 0.01 b | 0.173 ± 0.07 b | 0.071 ± 0.02 b | 0.057 ± 0.02 b | 0.005 ± 0.01 b |
Trifolium resupinatum L. | 12 | 0.072 ± 0.03 b | 0.202 ± 0.07 b | 0.011 ± 0.01 b | 0.218 ± 0.04 b | 0.057 ± 0.01 b | 0.051 ± 0.01 b | 0.004 ± 0.00 b |
Trifolium alexandrinum L. | 12 | 0.314 ± 0.20 b | 0.106 ± 0.06 b | 0.033 ± 0.02 b | 0.129 ± 0.02 b | 0.186 ± 0.10 b | 0.161 ± 0.09 a | 0.016 ± 0.01 b |
p | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | |
Wilting | ||||||||
Fresh forage | 24 | 0.928 ± 1.48 | 1.033 ± 1.59 | 0.056 ± 0.07 | 0.369 ± 0.36 | 0.165 ± 0.13 | 0.108 ± 0.07 | 0.046 ± 0.07 |
Wilted forage | 24 | 1.166 ± 1.90 | 1.349 ± 2.32 | 0.061 ± 0.08 | 0.363 ± 0.41 | 0.223 ± 0.25 | 0.142 ± 0.13 | 0.058 ± 0.10 |
p | 0.220 | 0.140 | 0.647 | 0.907 | 0.076 | 0.116 | 0.220 | |
Sampling | ||||||||
1. | 16 | 1.278 ± 2.06 | 1.413 ± 2.34 | 0.069 ± 0.09 | 0.430 ± 0.48 | 0.212 ± 0.26 | 0.136 ± 0.13 | 0.064 ± 0.10 |
2. | 16 | 1.153 ± 1.82 | 1.200 ± 1.98 | 0.061 ± 0.08 | 0.360 ± 0.37 | 0.200 ± 0.18 | 0.128 ± 0.09 | 0.058 ± 0.09 |
3. | 16 | 0.710 ± 1.10 | 0.960 ± 1.64 | 0.045 ± 0.05 | 0.308 ± 0.28 | 0.170 ± 0.15 | 0.110 ± 0.08 | 0.036 ± 0.06 |
p | 0.050 | 0.226 | 0.149 | 0.168 | 0.556 | 0.596 | 0.050 | |
Study plot | ||||||||
Vatín | 24 | 1.180 ± 1.82 | 1.421 ± 2.30 a | 0.060 ± 0.08 | 0.339 ± 0.32 | 0.202 ± 0.17 | 0.128 ± 0.09 | 0.059 ± 0.09 |
Troubsko | 24 | 0.914 ± 1.59 | 0.960 ± 1.60 b | 0.057 ± 0.08 | 0.393 ± 0.44 | 0.186 ± 0.22 | 0.122 ± 0.12 | 0.046 ± 0.08 |
p | 0.172 | 0.034 | 0.701 | 0.300 | 0.628 | 0.755 | 0.184 |
Statistically significant differences (p < 0.05) between means are indicated by different superscripts (a,b) within columns.