Table 1.
B. subtilis strains and plasmids used in this study.
| Strain | Relevant genotype | Reference or source |
| YB955 | hisC952 metB5 leuC427 xin-1 SpSENS | [26] |
| YB9801 | YB955 mfd::tet, Tet r | [22] |
| YB9900 | YB955 uvrA::em, Em r | a BSU35160 → YB955 |
| JJS164 | TF8a ΔpolA::sp, Sp r | [33] |
| AMP100 | YB955 ΔpolA::sp, Sp r | a JJS164 → YB955 |
| AMP101 | YB955 ΔpolA::sp, mfd::tet, Sp r Tet r | a JJS164 → YB9801 |
| BSU35160 | 168 uvrA::em, Em r | Bacillus Genetic Stock Center (BKE Knock-out collection) |
| HAM300 | YB955 uvrA::emmfd::tet, Em r Tet r | a YB9900 → YB9801 |
| PERM598 | 168 mutY::sp, Sp r | [31] |
| PERM687 | YB955 uvrA::sp, Sp r | Peter Setlow |
| PERM704 | YB955 mutY::sp, Sp r | [31] |
| PERM899 | YB955 mutY:: sp amyE::Phs-mutY, Cm r | [31] |
| PERM818 | YB955 mfd::tet mutY::sp, Tet r Sp r | a PERM598 → YB9801 |
| PERM995 | YB955 mfd::tet mutY::sp amyE::Phs-mutY, Cm r | b pPERM852 → PERM818 |
| PERM1029 | YB955 mutY::em, Em r | b pPERM979 → YB955 |
| PERM1041 | YB955 mfd::tet mutY::em, Tet r Em r | b pPERM979 → YB9801 |
| PERM1042 | YB955 mfd::tet mutY::em amyE::Phs-mfd, Tet r Em r Spr | b pPERM1041 → PERM1041 |
| PERM1046 | YB955 mfd::tet mutY::em amyE::Phs, Tet r Em r Sp r | b pdrE amyE → PERM1041 |
| PERM1352 | YB955 mutY::em uvrA::sp, Em r Sp r | a PERM1029 → PERM687 |
| PERM1353 | YB955 mfd::tet mutY::em uvrA::150, Tet r Em r | a PERM1029 → PERM1352 |
| PERM1123 | 168 pMUTIN4::mfd-lacZ, Em r. | [34] |
| Plasmids | Description | Reference or source |
| pHyperspank | Integrative vector, Sp r | David Rudner |
| pDR244 | cre, temperature-sensitive replication Amp r Sp r | Bacillus Genetic Stock Center (BKE Knock-out collection) |
| pPERM852 | pHyperspank-mutY Integrative vector with mutY control by IPTG, Amp r Cm r | [31] |
| pPERM979 | pMUTIN4::mutY, Amp r Em r | This study; [35] |
| pPERM1043 | pHyperspank-mfd Integrative vector with mfd under the control of IPTG, Amp r Sp r | This study |
| pPERM1084 | pBT-mutY, Cm r | This study |
| pPERM1072 | pTRG-mfd, Tet r | This study |
a Chromosomal DNA from the strain to left of the arrow was used to transform the strain to the right of the arrow; b DNA of the plasmid to the left of the arrow was used to transform the strain to the right of the arrow; Amp, ampicillin; Cm, chloramphenicol; Em, erythromycin; Neo, neomycin; Sp, spectinomycin; Tet, tetracycline; r Denotes phenotype of antibiotic resistance.