Figure 1.

Schematic work-flow of C. retropictus transcriptome using Illumina sequencing, de novo analysis, and annotation. The visceral mass transcriptome of C. retropictus was obtained using an Illumina HiSeq 2500 platform. The raw reads were pre-processed using the Sickle software tool (quality: 20, length: 40) and Fastq_filter software to obtain clean reads. Using Trinity (K-mer: 25; minimum contig length: 200) de novo assembler and TGICL clustering, the clean reads were processed to unigene sequences. Subsequently, the unigene sequences were blasted against public databases including PANM, Unigene, COG, GO, and KEGG for functional annotation. The SSRs were detected within the unigene sequences using MISA software.