Table 1.
Primer sequences and SSCP condition of SLA-DRA gene exons.
| Region | Location a | Primer F/R(5′→3′) | Amplification Fragment | Annealing Temperature (°C) | SSCP Condition |
|---|---|---|---|---|---|
| Exon 1 | 151–226 | F: CTTTGCTTGTATTGC R: ACCTAACTACCCCTC |
186 bp | 56.8 | 4 °C, 12%, 39:1, 190 V 20 h |
| Exon 3 | 4595–4876 | F: TGCTAAACAGGGAAGGCT R: ACAAAGGAGACTGAGGGATG |
352 bp | 56.8 | 4 °C, 10%, 39:1, 200 V 20 h |
| Exon 4 | 4155–4309 | F: TCCCGTAATACATCGTTC R: TTCCTTTCCTTGGCTCAT |
357 bp | 55.6 | 18 °C, 10%, 29:1, 200 V 18 h |
a Nucleotide positions refer to the SLA-DRA sequence in GenBank, accession No. AY 303990.