Table 2.
Results of the definitive bacterial reverse mutation assay on LMF-LJ.
| LMF-LJ (μg/mL) | S9 | Average Number of Revertants (Number of Colonies/Plate) | ||||
|---|---|---|---|---|---|---|
| Frameshift | Base Pair Substitution | Transition | ||||
| TA97a | TA98 | TA100 | TA1535 | TA102 | ||
| Negative control | – | 109.7 ± 7.2 a | 17.3 ± 2.9 | 102.0 ± 9.5 | 11.7 ± 1.5 | 475.0 ± 27.7 |
| 312.5 | – | 111.0 ± 5.5 | 14.3 ± 0.6 | 101.7 ± 11.5 | 12.3 ± 0.5 | 536.0 ± 31.4 |
| 625 | – | 123.3 ± 13.5 | 16.3 ± 4.5 | 101.7 ± 8.3 | 13.0 ± 2.6 | 541.3 ± 38.4 |
| 1250 | – | 128.3 ± 11.6 | 15.3 ± 5.8 | 109.0 ± 6.9 | 12.0 ± 3.0 | 494.7 ± 37.4 |
| 2500 | – | 114.0 ± 13.0 | 9.7 ± 1.5 | 103.0 ± 11.5 | 11.0 ± 3.0 | 480.7 ± 56.1 |
| 5000 | – | 112.0 ± 7.0 | 14.3 ± 1.1 | 104.0 ± 2.6 | 14.0 ± 3.0 | 432.0 ± 31.4 |
| Positive control | ||||||
| NPD | – | 524.7 ± 104.5 * | 836.0 ± 72.6 * | |||
| NaN3 | – | 1209.7 ± 263.3 * | 352.3 ± 45.6 * | |||
| MMC | – | 1970.7 ± 113.4 * | ||||
| Negative control | + | 138.3 ± 7.3 | 24.7 ± 1.5 | 122.0 ± 20.8 | 11.7 ± 1.5 | 503.0 ± 70.5 |
| 312.5 | + | 128.3 ± 16.0 | 27.0 ± 2.0 | 105.3 ± 8.1 | 12.0 ± 2.6 | 538.0 ± 14.0 |
| 625 | + | 136.0 ± 15.6 | 18.0 ± 4.3 | 123.0 ± 5.0 | 11.3 ± 4.6 | 516.7 ± 36.0 |
| 1250 | + | 152.3 ± 6.6 | 23.7 ± 1.5 | 121.7 ± 8.5 | 13.7 ± 0.5 | 510.7 ± 32.3 |
| 2500 | + | 123.3 ± 11.1 | 24.0 ± 3.4 | 108.0 ± 11.7 | 12.7 ± 3.2 | 485.3 ± 19.4 |
| 5000 | + | 128.0 ± 11.5 | 17.3 ± 4.9 | 111.3 ± 15.3 | 15.3 ± 2.5 | 452.0 ± 26.0 |
| Positive control | ||||||
| 2-AF | + | 367.3 ± 28.3 * | 287.3 ± 22.6 | |||
| BP | + | 59.7 ± 10.2 * | ||||
| 2-AA | + | 108.7 ± 15.5 * | 1009.3 ± 56.05 * | |||
a Values were expressed as mean ± SD, n = 3; * The number of revertant colonies was two-fold greater than the negative control. NPD: 4-nitro-o-phenylenediamine; MMC: mitomycin C; 2-AF: 2-aminofluorene; BP: benzo[α]pyrene; 2-AA: 2-aminoanthracene.