Table 3.
Astaxanthin, canthaxanthin, and β-carotene production by strains overexpressing various combinations of crtW and crtZ genes. Titers, productivities, and final ODs are given as means and standard deviations (n = 3) after 24 h of cultivation in CGXII + 100 mM glucose. B.a.: Brevundimonas aurantiaca; B.b.: Brevundimonas bacteroides; F.p.: Fulvimarina pelagi; S.a.: Sphingomonas astaxanthinifaciens.
| Strain Growth | Carotenoid Titer (mg·g−1·CDW) | Volumetric Productivity (mg·L−1·h−1) | |||||
|---|---|---|---|---|---|---|---|
| BETA4 Transformed with | Final OD600 nm | Astaxanthin | Canthaxanthin | β-Carotene | Astaxanthin | Canthaxanthin | β-Carotene |
| - | 28 ± 1 | <0.1 | <0.1 | 11.7 ± 2.0 | <0.1 | <0.1 | 3.4 ± 0.5 |
| (pSH1_crtWBb) (pEC-XT_crtZBb) | 21 ± 1 | <0.1 | <0.1 | 4.9 ± 0.4 | <0.1 | <0.1 | 1.1 ± 0.1 |
| (pSH1_crtWSa) (pEC-XT_crtZSa) | 22 ± 2 | < 0.1 | 0.3 ± 0.1 | 3.3 ± 0.5 | <0.1 | <0.1 | 0.8 ± 0.1 |
| (pSH1_crtWSa) (pEC-XT_crtZFp) | 24 ± 1 | 0.7 ± 0.3 | 0.2 ± 0.1 | 1.8 ± 0.1 | 0.2 ± 0.1 | <0.1 | 0.5 ± 0.1 |
| (pSH1_crtWBa) (pEC-XT_crtZFp) | 22 ± 1 | 1.7 ± 0.3 | 0.1 ± 0.1 | 2.0 ± 0.5 | 0.4 ± 0.1 | <0.1 | 0.4 ± 0.2 |
| (pSH1_crtWFp) (pEC-XT_crtZFp) = ASTA1 | 23 ± 1 | 1.6 ± 0.3 | 0.1 ± 0.1 | 0.3 ± 0.1 | 0.4 ± 0.1 | <0.1 | 0.1 ± 0.1 |