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Apoptosis/necrosis analysis was assessed using annexin-V FITC/PI differential staining. HCT-116 cells were exposed to 5 µM of compounds 3 (B), 5 (C) and 9 (D) for 24 h and compared to control cells (A). Cells positive FITC, PI or FITC/PI were determined using quadrant analysis and plotted as percent of total population (E). Data are expressed as Mean ± SEM, n = 3; * significantly different from corresponding control at p < 0.05.
