Figure 1. Localization of liposomes using near infrared fluoroscopy and immunohistochemistry.

(A,B) In vivo imaging using NIRF of the AVF directly after creation (day 0) in mice that were injected with PBS (A) or L-Pred. (B) Red color overlay corresponds to the intravenously administered methylene blue visualized on the 700 nm channel. Green color overlay corresponds to the intravenously administered liposomes that are labeled with the Alexa-750 fluorochrome visualized on the 800 nm channel. (C,D) In vivo imaging using NIRF of the AVF at time of sacrifice (day 14) in mice that were injected with either PBS (C) or L-Pred (D) at day 0, 2, 5 and 10. (*) Extravasation of liposomes in the anastomotic area of the AVF. (E) Double staining against F4/80 and gold particles showing accumulation of gold-labeled liposomes in macrophages in the venous outflow tract (black arrows). (a) Artery, (v) Vein. Bar = 10 μm.