Table 1. LC-MS/MS report of tryptic digested 6xHisFIP-Lrh.
| Accession number | Annotated protein name | Species | Molecular mass (Da)/Protein pI | Matched peptide fragments with annotated immunomodulatory protein (accession number 10641) from L. rhinocerotis genome database |
|---|---|---|---|---|
| 10641 | Immunomodulatory protein 8 | Ganoderma lucidum | 15759.4/9.01 | IQVYAVDPDTGNQFK |
| KKIQVYAVDPDTGNQFK | ||||
| GNPNNYIDNVVFPK | ||||
| KIQVYAVDPDTGNQFK | ||||
| FSVDYTPNWKR | ||||
| RGNPNNYIDNVVFPK | ||||
| VNFLEYNSGYGISDKKK | ||||
| VNFLEYNSGYGISDKK | ||||
| VNFLEYNSGYGISDK | ||||
| KYSYGVVVDGASLGVQAGYEVSSDGSQK | ||||
| YSYGVVVDGASLGVQAGYEVSSDGSQK | ||||
| IQVYAVDPDTGNQFKVAQWN | ||||
| GNPNNYIDNVVFPKVLTNK | ||||
| YSYGVVVDGASLGVQAGYEVSSDGSQKVNFLEYNSGYGISDK |
There were 14 distinct matched peptides, with distinct summed MS/MS Search score of 269.12 and a mean peptide spectral intensity of 3.21E+06.