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. 2016 Jul 27;6:29749. doi: 10.1038/srep29749

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Copyright © 2016, Macmillan Publishers Limited

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(A) Microscopic images of mutant PIK3CA knockin cells migration on collagen type I patterned nanogrooved PDMS substrates. Time-lapse video microscopy was used to capture the cellular motility and digital images were taken every 20 min for a total of 12 hrs per experiment. Scale bars = 100 μm. (B) The paths of individual migrating PIK3CA knockin cells on flat (B top) and nanogrooved (B bottom) migration on PDMS substrates was analyzed using custom MATLAB code. Color bar legend indicates migration speed of cells at each time-lapse in units of m/hr. (C) The migration direction of individual paths of PIK3CA knockin cells on both flat (C top) and nanogrooved (C bottom) substrates were measured and the portion of the migration directions within ±15 degree from the ECM patterns representing straight directionality of the migration is shown in each graph. As the ECM pattern widths became narrower from 120 μm to 30 μm, motile cells show greater straight directionality on both substrates with a decreased effect of nanotopography on migratory contact guidance.

Inline graphic — (A) Microscopic images of mutant PIK3CA knockin cells migration on collagen type I patterned nanogrooved PDMS substrates. Time-lapse video microscopy was used to capture the cellular motility and digital images were taken every 20 min for a total of 12 hrs per experiment. Scale bars = 100 μm. (B) The paths of individual migrating PIK3CA knockin cells on flat (B top) and nanogrooved (B bottom) migration on PDMS substrates was analyzed using custom MATLAB code. Color bar legend indicates migration speed of cells at each time-lapse in units of m/hr. (C) The migration direction of individual paths of PIK3CA knockin cells on both flat (C top) and nanogrooved (C bottom) substrates were measured and the portion of the migration directions within ±15 degree from the ECM patterns representing straight directionality of the migration is shown in each graph. As the ECM pattern widths became narrower from 120 μm to 30 μm, motile cells show greater straight directionality on both substrates with a decreased effect of nanotopography on migratory contact guidance.