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. 2016 Jul 27;6:30498. doi: 10.1038/srep30498

Figure 6. Internalized Tau alters stress granule dynamics and sensitizes cells to stress.

Figure 6

(A) Tau-GLuc-conditioned media or 0.1 mM Salubrinal was applied to HEK293T cells for 4 h, followed by a washout with normal media. Cells were fixed at 4 and 20 h after the washout and stained with TIA-1 and Tau-5 antibodies. DBeQ (10 μM) was used as a positive control to inhibit stress granule disassembly after the treatments. (B) Quantitation of immunofluorescence images: stress granule-positive cells over total cells were counted for each condition (n = 3). (C) Cell viability assay for the washout experiment (n = 4). (D,E) Cell viability assay for cells treated either with salubrinal (D) or with Tau-conditioned media (E) followed by 4 hours of recovery in fresh media and further treatement with 30 nM Rotenone for 6 h. In the salubrinal control cells Rotenone showed minimal toxicity while in cells exposed to Tau-GLuc-conditioned media Rotenone significantly decreased cell viability (n = 4). Scalebar = 10 μm; average +/− SEM is shown; ***p < 0.001.