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. 2016 Jul 26;35:120. doi: 10.1186/s13046-016-0396-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — CLDN6 expression is down-regulated drastically by DNA hypermethylation in breast cancer tissues and MCF-7 cells. a CLDN6 expression was significantly higher in breast pericarcinomatous tissues than that in breast cancer tissues by western blot. From lane 1 to lane 7 were represented of different tissue samples. P = 0.021 < 0.05. b The expression of CLDN6 in HBL-100、MCF-7 and COC1 cells (as tissue-specific control), determined by RT-PCR and Western blot assay. Differently with HBL-100 cells, CLDN6 expression was undetectable or low levels in MCF-7 cells. c CLDN6 DNA methylation status was determined in breast pericarcinomatous tissues and breast cancer tissues by MSP. (D) CLDN6 DNA methylation status was determined by MSP analysis in HBL-100, MCF-7 and COC1 cells. CLDN6, claudin-6; MSP, Methylation-Specific PCR