Table 1.

Primers for PCR assay

Gene name	Sequence (5’ → 3’)
Reverse transcription PCR primers
CLDN6
Sense	CACTGCCACTTCTGGATGG
Antisense	CAGTGCAGCTCCTTCAACCT
β-actin
Sense	CCACTGCGTCGCGGGG
Antisense	GGCAGCCAGCTCAGCCATG
GAPDH
Sense	TGTTGCCATCAATGACCCCTT
Antisense	CTCCACGACGTACTCAGCG
Methylation-Specific PCR primers
Unmethylated DNA
Sense	TGGATGTTTGTTAGTTTGAGGT
Antisense	ATAACCACAACCCAAATTCACA
Methylated DNA
Sense	ACGTTTGTTAGTTCGAGGC
Antisense	ATAACCGCAACCCGAATTC
ChIP primers
CLDN6
Sense	CCCATCCCCACGCACGCTT
Antisense	GCCACCTGGATGGGCGAGTC
Nuclease Accessibility primers
Primer1
Sense	TCTCCCCAAGGTGGTCTGCT
Antisense	CCTTTTCAAATCCAGGTTCGGC
Primer2
Sense	GGCGGTGGTCCAGTGACGT
Antisense	TAAAGGGAATAG AGGAAGCGTCC
Primer3
Sense	AGTCTCCCCAAGGTGGTCTGC
Antisense	CCTTTTCAAATCCAGGTTCGGC
Primer4
Sense	AGGAATTTCCCTTATCTCCTTCGC
Antisense	CCCATCCCCACGCACGCTT
Primer5
Sense	GCCACCTGGATGGGCGAGTC
Antisense	GAGGGTTCCCAATTTGCGGG
Primer6
Sense	CAGGGACAA AGACTCCGAAACC
Antisense	CTGGAACGCCGGTGACGT
Primer7
Sense	CTCCCCAAGGTGGTCTGCT
Antisense	TCAAATCCAGGTTCGGCATGTTA
Primer8
Sense	CCACTGCAGCTATAGAACCCCTCCA
Antisense	TTCTTTCCCCGTCCCCG