Table 1.
Primers used for drug resistant gene amplification
| Genes | Primers (Sequences 5’ → 3’) | Product size (bp) |
|---|---|---|
| katG | katG-F (5’-GACATTCGCGAGACGTTTCGG-3’) | 642 |
| katG-R (5’GCTCTTAAGGCTGGCAATCTCG-3’) | ||
| inhA | inhA-F (5’-CTATATCTCCGGTGCGGTCA-3’) | 469 |
| inhA-R (5’-CTTGGCCATCGAAGCATAC-3’) | ||
| inhA-Prom | inhA-Prom-1 (5’-TCCGTCATGGTCGAAGTGTG-3’) | 211 |
| inhA-Prom-2 (5’-GGTAACCAGGACTGAACGGG-3’) | ||
| rpoB | rpoB-F (5’-ACGGTCGGCGAGCTGATCC-3’) | 351 |
| rpoB-R (5’-CAGACCGATGTTGGGCCCCT-3’) | ||
| pncA | pncA-F1 (5’-GGCGTCATGGACCCTATATC-3’) | 670 |
| pncA-R1 (5’-CAACAGTTCATCCCGGTTC-3’) | ||
| embB | embB-1 (5’- TGATATTCGGCTTCCTGCTC-3’) | 417 |
| embB-2 (5’- ACCGCTCGATCAGCACATAG-3’) | ||
| ∆katG a | katGD-1 (5’-CCGGTCAAGAAGAAGTACGG-3’) | 590 |
| katGD-2 (5’-CTCTTCGTCAGCTCCCACTC-3’) | ||
| katGD-5 (5’-GGGGAACATCAAAGTGTCCT-3’) | 400 | |
| katGD-6 (5’-GATACCCATGTCGAGCAGGT-3’) |
aDeletion of katG